Characterization of protein profile in the Asian sea bass, Lates calcarifer (Bloch) exposed to copper

Abstract

Characterization of protein in the metabolically active tissues of muscle, gills and liver of the Asian sea bass, L. calcarifer on exposure to two sub-lethal doses (6.83 and 13.66 ppm) of copper were studied for 28 days of exposure (DoE). The electrophoretic pattern of muscle and gill proteins revealed 10 and 6 slow moving bands (control). The number of protein bands decreased to 7 and 6 in muscle, 5 and 4 in gills after 7 days of exposure to lower and higher concentrations of copper, respectively. After 28 days, the protein bands decreased to 6 and 5, 1 and 1in muscle and gill tissues at different concentrations of copper. In liver tissue protein concentration is very low so could not revealed any bands.