Effect of copper sulphate and pod extract of Acacia sinuata on biochemical constituent in freshwater snail Bellamya bengalensis

Abstract

Biochemical constituents (glycogen, protein and lipid) from animal body play vital role in its development. The quantity of these constituents is varied in accordance with exogenous and endogenous factors. In the present study, B. bengalensis was acutely (Mean LC50 concentrations of heavy metal of 0.56 ppm and pod extract 232 ppm) intoxicated with copper sulphate and A. sinuata. During exposure, the glycogen, protein and lipid concentration in different digestive organs were declined as per exposure period i.e. 24hrs, 48hrs, 72hrs and 96hrs. The targeted digestive organs of snail such as, salivary gland, oesophagus, intestine, stomach and hepatopancreas were subjected for biochemical determination. The maximum decline biochemical constituents were noted after 96 hours intoxication of copper sulphate and A. sinuata in digestive tract. The obtained results of biochemical revealed that, the stored nutrition found to be decreased with increase in the exposure period. Biochemical constituents from digestive tract found directly proportional to the time of exposure period. The decreased concentration and their nutritional values were discussed in relation to the physiology of digestion.