Effects of 2-phenoxyethanol anaesthesia on juvenile yellow tail anemone fish Amphiprion clarkii (Bennett, 1830), Pomacentridae

Abstract

The present research was performed to confirm the efficient concentration of the anaesthetic 2- phenoxyethanol (2-PE) on juvenile (1.3 ± 0.03g) yellow tail anemone fish (Amphiprion clarkii) and analysis the LC50 and LT50 of 2-PE at 28 ± 0.5oC, salinity 34 psu), pH 8.0-8.3 and dissolved oxygen 4-6 mg/l. The initiation point decreased and the revival moment increased with escalating concentrations. The results were conflict in revival moment and there were no significant differences among the revival moment from all concentrations. The most suitable concentration of 2-PE was 0.3 ml/l for about or over 10 min exposure time. The LC50 and LT50 for the 3-60 min exposure periods were estimated for juvenile A. clarkii. The toxic effect of 2-PE on survival rates of A. clarkii juveniles increased depending on the exposure period. In addition, 2-phenoxyethanol LT50 (median survival time) values, slope function (S) and lower and upper 95% confidence limits were estimated. The present work is applied first time in Indian aquaculture