The effect of Various kind of Live Feeds to Digestive Enzymes Activity of Coral Trout Plectropomus leopardus (Lacepède, 1802) larvae

Abstract

Digestive enzyme activity is a biological indicator of feed utilization by the larvae. The purpose of this study was to determine the digestive enzymes activity of coral trout larvae which fed (A) copepods, (B) copepods and rotifers and (C) rotifers. Biochemical analysis and SDS PAGE were performed to determine the enzymes activity and capability of protein hydrolysis on larvae and live feeds. The result indicates that the various kind of live feeds influence to digestive enzymes activity of coral trout larvae. Larvae B at 30 days after hatching had higher activity of protease, amylase and lipase (P<0.05) than larvae A and C. Larvae A and B had higher capability of protein hydrolysis than larvae C. Copepods had higher digestive enzymes activity (P<0.05) and higher capability of protein hydrolysis than rotifers. Therefore, feeding of copepods which combined with rotifers to coral trout larvae is strongly recommended.