Alachlor 50% EC induced biochemical alterations in Clarias batrachus during and after cessation of exposure

Abstract

The sub lethal effect of the Alachlor 50% EC on certain enzymes in tissue such as total protein, triglyceride, AST (Aspartate transaminase), ALT (Alanine transaminase) and ALP (Alkaline phosphatase) of the freshwater fish, Clarias batrachus during and after the cessation of the exposure were observed. Ten fish were exposed chronically to two sublethal concentrations for a period of 21 days and on completion of exposure five fish were sacrificed and tissues such as gill, liver and muscle were analysed for biochemical parameters and remaining five fish were maintained in clean well water for a period of seven days without test substance being spiked in the water and its tissue were collected after reversal period for biochemical analysis. Marked changes were observed in the biochemical parameters of the fish exposed and after the cessation of the exposure.