Kinetics of fiber digestion from in vitro gas production

Abstract

In vitro gas production, measured by computer-interfaced pressure sensors, was used to follow the digestion of a crystalline processed cellulose, a bacterial cellulose, and mixtures of these substrates by mixed ruminal bacteria. A first-order, substrate limited model (simple exponential with lag) and two bacterial growth models (logistic, Gompertz) were tested to fit these data. No single pool model gave an optimal fit to all substrates, but dual pool versions of both the logistic and Gompertz models fitted the data extremely well. Derivations of these models in the context of gas production are presented. The dual pool version of the exponential model commonly used to analyze fiber digestion was not able to reproduce the slope variations seen with mixed substrates. A modified dual pool logistic equation, with a single lag value, was selected to model the in vitro digestion of these substrates. The model was able to predict adequately both the input composition and the kinetic parameters for a defined mixture and gave a good fit (r2 > .995) to data from all the single and mixed substrates tested. This model may be useful for interpreting gas accumulation from natural feedstuffs.