Molecular detection of cutaneous leishmaniasis in human at district Hangu Khyber Pakhtunkhwa, Pakistan

Abstract

Cutaneous Leishmaniasis is parasitic disease which causes severe morbidity and mortality. The present study identified the leishmania infection caused by Leishmania tropica in Hangu. A total of 45 samples were examined through PCR, Microscopy which showed 87.61% (99/113), 53.98% (61/113) thus the sensitivity of PCR is very high as compared to microscopy. During the study 186 bp Leishmania tropica was identified through PCR and presence of Leishmania tropica showed the infected person serum through SDS method analysis respectively.