A Rapid, Simple, Sensitive Method for Measuring Fibrinolytic Split Products in Human Serum

Abstract

Summary and ConclusionsA simplified procedure for recognizing and quantitating fibrinolytic split products has been described. It utilizes human group O cells which can be sensitized and stored at 4° for 3 weeks and at ‐20° for many months prior to use. It is very sensitive, detecting 1.0 μg/ml of fibrinogen or split products. Application of the test to the Microtiter and Autotiter systems permits assay of many samples at once. Only 1 hr is required to complete the test and read the results. The interpretation of the quantitative assay result should be carefully made since, while fibrin split products are not absorbed from the sera during the preparation of the sample and are relatively accurately measured, fibrinogen split products tend to be absorbed during sample preparation and are thus less accurately measured. The test would appear to be more useful with fibrin lysis than with fibrinogen lysis and to be especially applicable in the diagnosis of defibrination syndrome.