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Parasite Lactate Dehydrogenase as an Assay for Plasmodium falciparum Drug Sensitivity

American Journal of Tropical Medicine and Hygiene · 1993 · Vol. 48(6) · pp. 739–741

Michael T. Makler✉(Portland VA Medical Center)Judith RiesJeffery A. WilliamsJ E BancroftRobert C. PiperBruce L. GibbinsDavid J. Hinrichs

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Abstract

This report compares the use of the lactate dehydrogenase (pLDH) assay with 3H-hypoxanthine incorporation and Giemsa microscopy for the evaluation of anti-malaria drug inhibition of the growth of P. falciparum in vitro. The inhibition profiles and IC50 determinations of the pLDH assay were directly comparable to those determined by the radioactive uptake and microscopic methods. Furthermore, the pLDH culture sensitivity assay is reproducible, easily interpreted, rapid and inexpensive to perform, suggesting field applicability.

Malaria Research and ControlLactate dehydrogenaseHypoxanthinePlasmodium falciparumL-Lactate dehydrogenaseDrugGiemsa stainBiologyMalariaColorimetryPharmacology

MeSH terms

AnimalsAzure StainsChloroquineColorimetryDose-Response Relationship, DrugDrug ResistanceHypoxanthinesL-Lactate DehydrogenasePlasmodium falciparumRegression AnalysisReproducibility of ResultsHypoxanthine

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742

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In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa