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Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides
Journal of Lipid Research · 1969 · Vol. 10(3) · pp. 341–344
Per Belfrage✉(National Institutes of Health)Martha Vaughan(National Institutes of Health)
Abstract
Oleic acid has a partition coefficient, upper phase/lower phase, of 1.9 (22 degrees C) in the liquid-liquid partition system described herein. Tri-, di-, and monoolein are found almost exclusively in the lower (organic) phase. Oleic acid can be quantitatively removed from mixtures of triglyceride and partial glycerides by means of this partition system under conditions resembling those in a lipase assay.
Metabolomics and Mass Spectrometry StudiesLipid metabolism and biosynthesisMicrobial Metabolic Engineering and BioproductionGlycerideOleic acidChromatographyChemistryIsolation (microbiology)Simple (philosophy)BiochemistryFatty acidBiologyBioinformatics
MeSH terms
AnimalsChemistry Techniques, AnalyticalEpididymisGlyceridesLipaseMaleMethodsOleic AcidsPhosphatidylcholinesTriglyceridesTrioleinTritiumRats
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766
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89%
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References
Removal of Fatty Acids from Serum Albumin by Charcoal Treatment
Journal of Biological Chemistry · 1967 · 2,102 citations
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