Determination of Total Phenol, Alkaloid, Flavonoid and Tannin in Different Extracts of Calanthe Triplicata.

Abstract

The present work was aimed to evaluate the total phenol, alkaloid, flavonoid and tannin contents in petroleum ether, chloroform, ethyl acetate and methanol extracts of Calanthe triplicata (Williemet) Ames. Gallic acid was used as standard for the determination of total phenol and tannin by Folin-Ciocalteu method. Total alkaloid content was determined chloride colorimetric method using quercetin as a standard. The results showed that ethyl acetate extract has high concentration of phenol, alkaloid, flavonoid and tannin contents as compared by bromocresol green solution using atropine as a standard. Total flavonoid content was determined by aluminium to petroleum ether, chloroform, ethyl acetate and methanol extracts. Ethyl acetate extract contained the total phenol of 29.43 and tannins of 82.92 as mg of gallic acid equivalents (GAE), alkaloids of 65.34 as mg of atropine equivalents (AE) and flavonoids of 90.24 as mg of quercetin equivalents (QE).