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A highly sensitive adenylate cyclase assay

Analytical Biochemistry · 1974 · Vol. 58(2) · pp. 541–548

Yoram Salomon✉(National Institute of Arthritis and Musculoskeletal and Skin Diseases)Constantine Londos(National Institute of Arthritis and Musculoskeletal and Skin Diseases)Martin Rodbell(National Institutes of Health)

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Chemical Synthesis and Analysis Enzyme Structure and Function RNA and protein synthesis mechanismsAdenylate kinaseCyclaseChemistryBiochemistryEnzyme

MeSH terms

Adenylyl CyclasesAluminumAnimalsCell MembraneChromatographyChromatography, Ion ExchangeEvaluation Studies as TopicGlucagonKineticsLiverMethodsMicrochemistryPhosphorus RadioisotopesTime FactorsRats

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Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.