articleTop 10% cited
Use of Polyethylene Glycol to Separate Free and Antibody-Bound Peptide Hormones in Radioimmunoassays†
The Journal of Clinical Endocrinology & Metabolism · 1971 · Vol. 33(5) · pp. 732–738
Bernard Desbuquois✉(National Institutes of Health)G D Aurbach(National Institutes of Health)
Abstract
Aqueous polyethylene glycol causes precipitation of antibody-bound peptide hormones labeled with radioactive iodine with little or no precipitation of free hormones. Based on this finding, a method of separation has been developed and applied to radioimmunoassays of insulin, parathyroid hormone, growth hormone and arginine vasopressin. The new method provides several advantages over the double-antibody precipitation method. It appears particularly valuable in immunoassays of substances of low molecular weight.
Ion channel regulation and functionParticle accelerators and beam dynamicsRadiopharmaceutical Chemistry and ApplicationsRadioimmunoassayPolyethylene glycolVasopressinHormoneChemistryPrecipitationPeptide hormonePeptideAntibodyChromatography
MeSH terms
AnimalsAntigen-Antibody ComplexCattleGlycolsGuinea PigsHumansInsulinIodine IsotopesMethodsParathyroid HormoneChemical PrecipitationRadioimmunoassayGrowth HormoneSwineVasopressins
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References
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Journal of Chromatography A · 1973 · 2,011 citations
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