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Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates

Journal of Antimicrobial Chemotherapy · 2007 · Vol. 60(2) · pp. 394–397
Vincent CattoirLaurent PoirelVincent O. RotimiClaude-James SoussyPatrice Nordmann

Abstract

We report here a fast and reliable technique for rapid screening of qnr-positive strains to be used for epidemiological surveys. A low prevalence of Qnr determinants among ESBL-producing Enterobacteriaceae was identified in the study with Kuwaiti isolates.

Antibiotic Resistance in BacteriaEnterobacteriaceae and Cronobacter ResearchProbiotics and Fermented FoodsQuinolonePlasmidMultiplex polymerase chain reactionMicrobiologyBiologyGeneCiprofloxacinDNA gyraseMultiplexAntibacterial agent

MeSH terms

Anti-Bacterial Agentsbeta-LactamasesEnterobacteriaceaeEnterobacteriaceae InfectionsHumansKuwaitMicrobial Sensitivity TestsPlasmidsQuinolonesDNA PrimersReverse Transcriptase Polymerase Chain ReactionDrug Resistance, BacterialEscherichia coli Proteins

Funding

  • Institut National de la Santé et de la Recherche Médicale
Citations
678
FWCI
10.61
field-weighted impact
References
9
Percentile
99%
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Citations per year
References
The worldwide emergence of plasmid-mediated quinolone resistance
The Lancet Infectious Diseases · 2006 · 964 citations
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