articleTop 10% cited
Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
Gene · 1995 · Vol. 164(1) · pp. 49–53
Willem P.C. Stemmer✉(Affymax (United States))Andreas Crameri(Affymax (United States))Kim D. HaThomas M. BrennanHerbert L. Heyneker
Bacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsCRISPR and Genetic EngineeringBiologyMolecular biologyPlasmidOligonucleotideTransformation (genetics)GeneRestriction enzymeDNAIn vitro recombinationOverlap extension polymerase chain reaction
MeSH terms
beta-LactamasesDNA-Directed DNA PolymeraseGenes, SyntheticOligodeoxyribonucleotidesPlasmidsPolymerase Chain Reaction
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References
Randomization of genes by PCR mutagenesis.
Genome Research · 1992 · 1,071 citations
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