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Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination

Biophysical Journal · 2008 · Vol. 94(12) · pp. 4957–4970

Mats G. Gustafsson✉(University of California, San Francisco)Lin Shao(University of California, San Francisco)Peter M. Carlton(University of California, San Francisco)Rachel Wang(University of California, Berkeley)Inna Golubovskaya(University of California, Berkeley)W. Zacheus Cande(University of California, Berkeley)David A. Agard(Howard Hughes Medical Institute)John W. Sedat(University of California, San Francisco)

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Advanced Fluorescence Microscopy Techniques Advanced Electron Microscopy Techniques and Applications Near-Field Optical MicroscopyMicroscopyOpticsResolution (logic)Light sheet fluorescence microscopyGratingOptical microscopeImage resolutionSuper-resolution microscopyMaterials scienceFluorescence microscope

MeSH terms

AlgorithmsImage EnhancementImage Interpretation, Computer-AssistedLightingMicroscopy, FluorescenceSensitivity and SpecificityReproducibility of ResultsImaging, Three-Dimensional

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Super-Resolution Fluorescence Microscopy