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Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish

Developmental Cell · 2014 · Vol. 32(1) · pp. 97–108

Fatma O. Kok(University of Massachusetts Chan Medical School)Masahiro Shin(University of Massachusetts Chan Medical School)Chih‐Wen Ni(University of Massachusetts Chan Medical School)Ankit Gupta(University of Massachusetts Chan Medical School)Ann S. Grosse(University of Massachusetts Chan Medical School)Andreas van Impel(Hubrecht Institute for Developmental Biology and Stem Cell Research)Bettina C. Kirchmaier(Hubrecht Institute for Developmental Biology and Stem Cell Research)Josi Peterson-Maduro(Hubrecht Institute for Developmental Biology and Stem Cell Research)George Kourkoulis(University of Massachusetts Chan Medical School)Ira Male(University of Massachusetts Chan Medical School)Dana F. DeSantis(University of Massachusetts Chan Medical School)Sarah Sheppard-Tindell(University of Massachusetts Chan Medical School)Lwaki Ebarasi(Karolinska Institutet)Christer Betsholtz(Karolinska Institutet)Stefan Schulte‐Merker(University of Münster)Scot A. Wolfe(University of Massachusetts Chan Medical School)Nathan D. Lawson✉(University of Massachusetts Chan Medical School)

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CRISPR and Genetic Engineering Zebrafish Biomedical Research Applications Pluripotent Stem Cells ResearchZebrafishBiologyMorpholinoMutantPhenotypeGeneticsGeneSanger sequencingMutation

MeSH terms

AnimalsDeoxyribonucleasesGene Expression RegulationMutationPhenotypeRNA, MessengerZebrafishBlotting, WesternOligonucleotides, AntisenseGene Expression Regulation, DevelopmentalReverse Transcriptase Polymerase Chain ReactionZebrafish ProteinsGene Knockdown TechniquesMorpholinosReal-Time Polymerase Chain Reaction

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