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Detection of erythromycin-resistant determinants by PCR

Antimicrobial Agents and Chemotherapy · 1996 · Vol. 40(11) · pp. 2562–2566

Joyce A. Sutcliffe✉(Pfizer (United States))T Grebe✉(Pfizer (United States))Amelia Tait‐Kamradt✉(Pfizer (United States))L Wondrack✉(Pfizer (United States))

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Abstract

Erythromycin resistance determinants include Erm methylases, efflux pumps, and inactivating enzymes. To distinguish the different mechanisms of resistance in clinical isolates, PCR primers were designed so that amplification of the partial gene products could be detected in multiplex PCRs. This methodology enables the direct sequencing of amplified PCR products that can be used to compare resistance determinants in clinical strains. Further, this methodology could be useful in surveillance studies of erythromycin-resistant determinants.

Antibiotic Resistance in Bacteria Mycobacterium research and diagnosis Pneumocystis jirovecii pneumonia detection and treatmentErythromycinMultiplex polymerase chain reactionBiologyEffluxGeneMicrobiologyMultiplexPolymerase chain reactionLincosamidesDrug resistance

MeSH terms

Amino Acid SequenceAnti-Bacterial AgentsBacteriaDrug Resistance, MicrobialErythromycinHumansMolecular Sequence DataPolymerase Chain ReactionGram-Positive Bacterial InfectionsDNA Primers

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993

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7.38

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Nomenclature for Macrolide and Macrolide-Lincosamide-Streptogramin B Resistance Determinants