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Escherichia coli Physiology in Luria-Bertani Broth

Journal of Bacteriology · 2007 · Vol. 189(23) · pp. 8746–8749

Guennadi Sezonov✉(Institut Jacques Monod)Danièle Joseleau‐Petit(Institut Jacques Monod)Richard D’Ari(Institut Jacques Monod)

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Abstract

Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.

Bacterial Genetics and Biotechnology Microbial Metabolic Engineering and Bioproduction Evolution and Genetic DynamicsBiologyEscherichia coliMicrobiologyEnterobacteriaceaeBacteriologyBacteriaBiochemistryGenetics

MeSH terms

Amino AcidsCarbohydratesCarbonCulture MediaEscherichia coliFermentationCell Proliferation

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<i>Escherichia coli</i> Physiology in Luria-Bertani Broth

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