article Open AccessTop 10% cited
Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons
Clinical Chemistry · 2004 · Vol. 50(7) · pp. 1156–1164
Michael Liew✉(ARUP Laboratories (United States))Robert J. Pryor(University of Utah)Robert Palais(University of Utah)Cindy Meadows(ARUP Laboratories (United States))Maria Erali(ARUP Laboratories (United States))Elaine Lyon(ARUP Laboratories (United States))Carl T. Wittwer(University of Utah)
Abstract
SNP genotyping by high-resolution melting analysis is simple, rapid, and inexpensive, requiring only PCR, a DNA dye, and melting instrumentation. The method is closed-tube, performed without probes or real-time PCR, and can be completed in less than 2 min after completion of PCR.
Molecular Biology Techniques and ApplicationsCRISPR and Genetic EngineeringRNA Interference and Gene DeliveryAmpliconSingle-nucleotide polymorphismMelting curve analysisGenotypingGenotypeHigh Resolution MeltMolecular biologyBiologyGeneticsMethylenetetrahydrofolate reductase
MeSH terms
Factor VGenotypeHemochromatosisHumansMutationProthrombinPolymerase Chain ReactionPolymorphism, Single NucleotideMethylenetetrahydrofolate Reductase (NADPH2)
Funding
- University of Utah
Citations
655
FWCI
6.73
field-weighted impact
References
42
Percentile
98%
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Citations per year
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References
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Genome Research · 1995 · 1,606 citations
High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen
Clinical Chemistry · 2003 · 1,248 citations
The Sequence of the Human Genome
Science · 2001 · 13,619 citations
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