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Electrophoretic resolution of the ‘major outer membrane protein’ of <i>Escherichia coli</i> K12 into four bands
FEBS Letters · 1975 · Vol. 58(1-2) · pp. 254–258
Ben Lugtenberg✉(Utrecht University)Jos Meijers(Utrecht University)R.C. Peters(Utrecht University)Peter van der Hoek(Utrecht University)Loek van Alphen(Utrecht University)
Abstract
The cell envelope of Enterobacteriaceae consists of \ntwo membranes separated by a peptidoglycan layer. \nMethods have been developed to separate the cytoplasmic \nmembrane from the outer membrane. \nThe outer membrane of Escherichia coli contains 60% \nof the envelope protein. Using polyacrylamide \ngelelectrophoresis, Schnaitman found six protein \nbands in the outer membrane fraction, of which one \nband (mol. wt 44 000) accounted for 70% of the total \nouter membrane protein.
Lipid Membrane Structure and BehaviorBacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsClinical microbiologyLibrary sciencePhysicsBiologyMicrobiologyComputer science
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References
Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System
Journal of Biological Chemistry · 1971 · 1,999 citations
Chemical Characterization, Spatial Distribution and Function of a Lipoprotein (Murein-Lipoprotein) of the E. coli Cell Wall. The Specific Effect of Trypsin on the Membrane Structure
European Journal of Biochemistry · 1969 · 543 citations
Analysis of bacteriophage T7 early RNAs and proteins on slab gels
Journal of Molecular Biology · 1973 · 2,228 citations
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature · 1970 · 251,243 citations
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