article Open Access

Multilocus Sequence Typing of Klebsiella pneumoniae Nosocomial Isolates

Journal of Clinical Microbiology · 2005 · Vol. 43(8) · pp. 4178–4182

Laure Diancourt✉(Institut Pasteur)Virginie Passet(Institut Pasteur)J. Verhoef(University Medical Center Utrecht)Patrick A. D. Grimont(Institut Pasteur)Sylvain Brisse(Inserm)

View via DOI Publisher page

Abstract

A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.

Antibiotic Resistance in Bacteria Genomics and Phylogenetic Studies Pneumonia and Respiratory InfectionsMultilocus sequence typingKlebsiella pneumoniaeHousekeeping geneBiologyMicrobiologyTypingRibotypingMolecular epidemiologyGeneticsGenotype

MeSH terms

Base SequenceCross InfectionHumansKlebsiella pneumoniaeMicrobial Sensitivity TestsMolecular Sequence DataBacterial Typing TechniquesSequence Analysis, DNARibotypingDrug Resistance, Bacterial

Citations

1,346

FWCI

2.04

field-weighted impact

References

Percentile

85%

vs. same field & year

Citations per year

Cited by

Tracking a Hospital Outbreak of Carbapenem-Resistant Klebsiella pneumoniae with Whole-Genome Sequencing