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Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery

PLoS ONE · 2010 · Vol. 5(12) · pp. e15004–e15004

Larry Gold✉(University of Colorado Boulder)Deborah Ayers(SomaLogic (United States))Jennifer Bertino(SomaLogic (United States))C. Bock(SomaLogic (United States))Ashley Bock(SomaLogic (United States))Edward N. Brody(SomaLogic (United States))Jeff Carter(SomaLogic (United States))Andrew B Dalby(SomaLogic (United States))Bruce E. Eaton(University of Colorado Boulder)Tim Fitzwater(SomaLogic (United States))Dylan Flather(SomaLogic (United States))Ashley Forbes(SomaLogic (United States))Trudi Foreman(SomaLogic (United States))Cate Fowler(SomaLogic (United States))Bharat Gawande(SomaLogic (United States))Meredith Goss(SomaLogic (United States))Magda Gunn(SomaLogic (United States))Shashi Kumar Gupta(SomaLogic (United States))Dennis Halladay(SomaLogic (United States))Jim Heil(SomaLogic (United States))Joe Heilig(SomaLogic (United States))Brian J. Hicke(SomaLogic (United States))Gregory M. Husar(SomaLogic (United States))Nebojša Janjić(SomaLogic (United States))Thale C. Jarvis(SomaLogic (United States))Susan Jennings(SomaLogic (United States))Evaldas Katilius(SomaLogic (United States))Tracy R. Keeney(SomaLogic (United States))Nancy Kim(SomaLogic (United States))Tad H. Koch(University of Colorado Boulder)Stephan Kräemer(SomaLogic (United States))Luke Kroiss(SomaLogic (United States))Ngan Le(SomaLogic (United States))Daniel M. Levine(The Rogosin Institute)Wes Lindsey(SomaLogic (United States))Bridget Lollo(SomaLogic (United States))Wes Mayfield(SomaLogic (United States))Mike Mehan(SomaLogic (United States))Robert Mehler(SomaLogic (United States))S. Kim Nelson(SomaLogic (United States))Michele Nelson(SomaLogic (United States))Dan Nieuwlandt(SomaLogic (United States))Malti Nikrad(SomaLogic (United States))Urs A. Ochsner(SomaLogic (United States))Rachel Ostroff(SomaLogic (United States))Matt Otis(SomaLogic (United States))Thomas S. Parker(The Rogosin Institute)Steve Pietrasiewicz(SomaLogic (United States))Daniel I. Resnicow(SomaLogic (United States))John C. Rohloff(SomaLogic (United States))Glenn M. Sanders(SomaLogic (United States))Sarah Sattin(SomaLogic (United States))Daniel J. Schneider(SomaLogic (United States))Britta Swebilius Singer(SomaLogic (United States))Martin Stanton(SomaLogic (United States))Alana Sterkel(SomaLogic (United States))Alexandre F.R. Stewart(SomaLogic (United States))Suzanne Stratford(SomaLogic (United States))Jonathan D. Vaught(SomaLogic (United States))Mike Vrkljan(SomaLogic (United States))Jeffrey J. Walker✉(SomaLogic (United States))Mike Watrobka(SomaLogic (United States))Sheela Waugh(SomaLogic (United States))Allison Weiss(SomaLogic (United States))Sheri K. Wilcox(SomaLogic (United States))Alexey Wolfson(SomaLogic (United States))Steven K. Wolk(SomaLogic (United States))Chi Zhang(SomaLogic (United States))Dom Zichi(SomaLogic (United States))

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Abstract

We describe a versatile and powerful tool that allows large-scale comparison of proteome profiles among discrete populations. This unbiased and highly multiplexed search engine will enable the discovery of novel biomarkers in a manner that is unencumbered by our incomplete knowledge of biology, thereby helping to advance the next generation of evidence-based medicine.

Advanced biosensing and bioanalysis techniques Advanced Biosensing Techniques and Applications Advanced Proteomics Techniques and ApplicationsAptamerBiomarker discoveryProteomeProteomicsComputational biologyBiomarkerDNA microarrayOligonucleotideBiologyProtein microarray

MeSH terms

AgedFemaleGenetic TechniquesGlomerular Filtration RateHumansKidney Failure, ChronicKineticsMaleMiddle AgedNucleic Acid HybridizationMass SpectrometryReproducibility of ResultsBiomarkersGene LibraryEvidence-Based Medicine