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Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

Analytical Biochemistry · 1980 · Vol. 109(1) · pp. 76–86
Dayle A. HagerRichard R. Burgess
DNA and Nucleic Acid ChemistryBacteriophages and microbial interactionsBacterial Genetics and BiotechnologySodium dodecyl sulfateChemistryGel electrophoresisChromatographyPolyacrylamide gel electrophoresisSodiumBiochemistryEnzyme

MeSH terms

AcetoneDNA Topoisomerases, Type IElectrophoresis, Polyacrylamide GelEscherichia coliMicrochemistryMolecular WeightPlantsProteinsDNA-Directed RNA PolymerasesSigma FactorSodium Dodecyl SulfateTranscription Factors
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