articleTop 1% cited
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes
Analytical Biochemistry · 1980 · Vol. 109(1) · pp. 76–86
Dayle A. Hager✉(University of Wisconsin–Madison)Richard R. Burgess(University of Wisconsin–Madison)
DNA and Nucleic Acid ChemistryBacteriophages and microbial interactionsBacterial Genetics and BiotechnologySodium dodecyl sulfateChemistryGel electrophoresisChromatographyPolyacrylamide gel electrophoresisSodiumBiochemistryEnzyme
MeSH terms
AcetoneDNA Topoisomerases, Type IElectrophoresis, Polyacrylamide GelEscherichia coliMicrochemistryMolecular WeightPlantsProteinsDNA-Directed RNA PolymerasesSigma FactorSodium Dodecyl SulfateTranscription Factors
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References
Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography
Biochemistry · 1975 · 1,149 citations
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Nature · 1970 · 251,243 citations
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