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Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector

Gene · 1986 · Vol. 48(1) · pp. 119–131

Jens P. Fürste(Max Planck Institute for Molecular Genetics)Werner Pansegrau(Max Planck Institute for Molecular Genetics)Ronald FrankHelmut BlöckerPeter Scholz(Max Planck Institute for Molecular Genetics)Michael Bagdasarian(Max Planck Institute for Molecular Genetics)Erich Lanka✉(Max Planck Institute for Molecular Genetics)

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Bacterial Genetics and Biotechnology Bacteriophages and microbial interactions RNA and protein synthesis mechanismsBiologyPlasmidMultiple cloning siteRepliconCloning vectorColE1Expression vectorRepressorMolecular biologyGenetics

MeSH terms

Cloning, MolecularEscherichia coliGene Expression RegulationGenes, BacterialGenetic VectorsGram-Negative BacteriaPlasmidsPromoter Regions, GeneticRepliconRNA NucleotidyltransferasesDNA Primase

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Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli