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Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Biochemistry · 1979 · Vol. 18(24) · pp. 5294–5299
John M. Chirgwin✉(Washington University in St. Louis)Alan PrzybylaRaymond J. MacDonaldWilliam J. Rutter(University of California, San Francisco)
Abstract
Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of alpha-amylase messenger ribonucleic acid.
DNA and Nucleic Acid ChemistryAnalytical Chemistry and ChromatographyProtein Structure and DynamicsRNARibonucleaseRNase PChemistryBiochemistryHomogenization (climate)Pancreatic ribonucleaseMolecular biologyBiology
MeSH terms
AnimalsMethioninePancreasRabbitsReticulocytesRibonucleasesRNARNA, MessengerProtein BiosynthesisRats
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References
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
Biochemical Journal · 1956 · 14,130 citations
An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates
European Journal of Biochemistry · 1976 · 3,966 citations
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acid
Biochemistry · 1974 · 965 citations
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
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