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An automated procedure for the determination of soluble carbohydrates in herbage

Journal of the Science of Food and Agriculture · 1977 · Vol. 28(7) · pp. 639–642
T. A. Thomas

Abstract

Abstract An automated procedure for the estimation of total soluble carbohydrate in water extracts of herbage is described. Addition of different sugars to grass extracts showed good recoveries (96.5–104.5%) for glucose, fructose, sucrose and fructosan. Analysis of sugar mixtures similar in composition to grass extracts also gave good recoveries (98.7–100%). The automated technique enabled 20 samples per hour to be analysed with a lower coefficient of variation (1.96%) than for a manual anthrone method (3.85%).

Analytical Chemistry and ChromatographyMicrobial Metabolites in Food BiotechnologyChromatography in Natural ProductsAnthroneFructoseSucroseSugarChemistryChromatographyCarbohydrateCoefficient of variationComposition (language)Food science
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References
The estimation of carbohydrates in plant extracts by anthrone
Biochemical Journal · 1954 · 4,282 citations
Chemistry and biochemistry of herbage
Phytochemistry · 1974 · 738 citations
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