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Improved single and multicopy lac-based cloning vectors for protein and operon fusions
Gene · 1987 · Vol. 53(1) · pp. 85–96
Robert W. Simons✉(Harvard University)Fariba Houman(Harvard University)Nancy Kleckner(Harvard University)
Bacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsCRISPR and Genetic EngineeringBiologyOperonlac operonPlasmidGeneticsProphageGeneCloning (programming)TransposaseTranscription (linguistics)
MeSH terms
Gene Expression RegulationGenetic VectorsLac OperonOperonRecombinant Fusion ProteinsRecombinant ProteinsTranscription, GeneticProtein Biosynthesis
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References
In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals
Journal of Bacteriology · 1980 · 1,047 citations
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
Journal of Molecular Biology · 1981 · 1,722 citations
Experiments with gene fusions
Trends in Genetics · 1985 · 1,214 citations
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