article Open AccessTop 10% cited

Two New Nuclear Isolation Buffers for Plant DNA Flow Cytometry: A Test with 37 Species

Annals of Botany · 2007 · Vol. 100(4) · pp. 875–888

João Loureiro✉(University of Aveiro)Eleazar Rodriguez(University of Aveiro)Jaroslav Doležel(Czech Academy of Sciences, Institute of Experimental Botany)Conceição Santos(University of Aveiro)

View via DOI Full-text PDF

Abstract

WPB is a reliable buffer which is also suitable for the analysis of problematic tissues/species. Although GPB failed with some plant species, it provided high-quality DNA histograms in species from which nuclear suspensions are easy to prepare. The results indicate that even with a broad range of species, either GPB or WPB is suitable for preparation of high-quality suspensions of intact nuclei suitable for DNA flow cytometry.

Plant Pathogens and Resistance Plant Disease Resistance and Genetics Chromosomal and Genetic VariationsBiologyNuclear DNAPropidium iodideFlow cytometryHerbaceous plantDNADNA extractionBotanyMolecular biologyBiochemistry