articleTop 10% cited
High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
Nature Protocols · 2007 · Vol. 2(1) · pp. 31–34
R. Daniel Gietz✉Robert H. Schiestl(UCLA Health)
CRISPR and Genetic EngineeringFungal and yeast genetics researchViral Infectious Diseases and Gene Expression in InsectsTransformation (genetics)Transformation efficiencyYeastSaccharomyces cerevisiaePEG ratioYield (engineering)DNALithium (medication)Computational biologyChemistry
MeSH terms
AcetatesDNA, Single-StrandedGenetic TechniquesHot TemperaturePolyethylene GlycolsSaccharomyces cerevisiaeTransformation, Genetic
Citations
2,897
FWCI
8.44
field-weighted impact
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10
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98%
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Citations per year
References
Transformation of intact yeast cells treated with alkali cations
Journal of Bacteriology · 1983 · 7,672 citations
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