articleTop 10% cited
AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINS
Transplantation · 1968 · Vol. 6(6) · pp. 761–764
William Boyle✉(Duke University Hospital)
Abstract
SUMMARY The 51Cr-release assay for measurement of cytotoxic reactions, with its many advantages, has hitherto been applicable only to mouse cell types which occur free from contamination with erythrocytes. A simple method is presented which can selectively lyse contaminating erythrocytes from tissue cell preparations without damaging the tissue cells. The tissue cells can then be labelled and tested by the 51Cr-release cytotoxicity assay. The usefulness of the method is shown by demonstrating its success with suspensions of mouse spleen cells and with a hemorrhagic ascites sarcoma.
Immunotoxicology and immune responsesAnimal testing and alternativesCarcinogens and Genotoxicity AssessmentCytotoxicityLysisSpleenCytotoxic T cellCellChemistryMolecular biologyAscitesIn vitroNeoplasm
MeSH terms
AnimalsAntigen-Antibody ReactionsChromium IsotopesIsoantibodiesMethodsSarcoma, ExperimentalSpleenMice
Citations
701
FWCI
4.99
field-weighted impact
References
0
Percentile
95%
vs. same field & year
Citations per year
Cited by
Immunofluorescent studies of the development of pre‐B cells, B lymphocytes and immunoglobulin isotype diversity in humans
European Journal of Immunology · 1977 · 487 citations
Citation Network
How this paper connects to the literature. Drag to explore, click any node to open that paper.