article
An improved colour reagent for the determination of blood glucose by the oxidase system
The Analyst · 1972 · Vol. 97(1151) · pp. 142–142
Abstract
A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.
Electrochemical sensors and biosensorsAnalytical Chemistry and SensorsPhotoreceptor and optogenetics researchGlucose oxidaseReagentChemistryChromatographyOxidase testBiochemistryEnzymeOrganic chemistry
MeSH terms
Blood GlucoseColorimetryGlucose OxidaseIndicators and ReagentsMethodsPhenazinesPhenols
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