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A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250
Analytical Biochemistry · 1977 · Vol. 79(1-2) · pp. 544–552
J. J. Sedmak✉(Medical College of Wisconsin)Sidney E. Grossberg(Medical College of Wisconsin)
Meat and Animal Product QualityProtein purification and stabilityMass Spectrometry Techniques and ApplicationsCoomassie Brilliant BlueAbsorbanceReagentChromatographyChemistryBradford protein assayBicinchoninic acid assayLowry protein assayReproducibilityOrange (colour)
MeSH terms
BuffersMethodsMolecular WeightPeptidesProteinsRosaniline DyesSerum Albumin, BovineStaining and Labeling
Funding
- U.S. Public Health Service
- National Institute of Allergy and Infectious Diseases
Citations
3,013
FWCI
42.27
field-weighted impact
References
12
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100%
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Citations per year
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References
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Journal of Biological Chemistry · 1951 · 317,666 citations
Fluorometric assay of proteins in the nanogram range
Archives of Biochemistry and Biophysics · 1973 · 1,634 citations
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Analytical Biochemistry · 1973 · 2,523 citations
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