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Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

Gene · 1995 · Vol. 158(1) · pp. 9–14
Peter CherepanovWilfried Wackernagel
Bacterial Genetics and BiotechnologyCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsBiologyGeneticsFLP-FRT recombinationRecombinaseGenePlasmidEscherichia coliHomologous recombinationMutantSite-specific recombination

MeSH terms

CatalysisDNA Transposable ElementsDNA NucleotidyltransferasesEscherichia coliKanamycin ResistancePlasmidsRecombination, GeneticTetracycline ResistanceGene ExpressionMutagenesis, InsertionalSequence Deletion
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Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant · Scinovex