article
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant
Gene · 1995 · Vol. 158(1) · pp. 9–14
Peter Cherepanov(Carl von Ossietzky Universität Oldenburg)Wilfried Wackernagel✉(Carl von Ossietzky Universität Oldenburg)
Bacterial Genetics and BiotechnologyCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsBiologyGeneticsFLP-FRT recombinationRecombinaseGenePlasmidEscherichia coliHomologous recombinationMutantSite-specific recombination
MeSH terms
CatalysisDNA Transposable ElementsDNA NucleotidyltransferasesEscherichia coliKanamycin ResistancePlasmidsRecombination, GeneticTetracycline ResistanceGene ExpressionMutagenesis, InsertionalSequence Deletion
Citations
1,989
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2.26
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25
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88%
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References
Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5
Gene · 1982 · 934 citations
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
Journal of Bacteriology · 1978 · 4,277 citations
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