articleTop 10% cited

Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning

Journal of Immunological Methods · 2007 · Vol. 329(1-2) · pp. 112–124

Thomas Tiller(Max Planck Institute for Infection Biology)Eric Meffre(Hospital for Special Surgery)Sergey Yurasov(Memorial Sloan Kettering Cancer Center)Makoto Tsuiji(Tokyo Medical and Dental University)Michel C. Nussenzweig(Howard Hughes Medical Institute)Hedda Wardemann✉(Max Planck Society)

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Monoclonal and Polyclonal Antibodies Research T-cell and B-cell Immunology CAR-T cell therapy researchMonoclonal antibodyMolecular biologyclone (Java method)BiologyCloning (programming)AntibodyCell sortingRecombinant DNAImmunoglobulin light chainAntibody Repertoire

MeSH terms

Antibodies, MonoclonalAntibody SpecificityB-LymphocytesBase SequenceCell LineCell SeparationCells, CulturedCloning, MolecularCross ReactionsFlow CytometryGenetic VectorsHumansImmunoglobulin GImmunoglobulin Heavy ChainsImmunoglobulin Light Chains

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1,144

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98%

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References

Sequences of proteins of immunological interest

Analytical Biochemistry · 1984 · 3,390 citations

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