Use of Mycoplasmalike Organism (MLO) Group-Specific Oligonucleotide Primers for Nested-PCR Assays to Detect Mixed-MLO Infections in a Single Host Plant

Abstract

Oligonucleotide primer pairs R16(I)F1/R1, R16(III)F2/R1, and R16(V)F1/Ri for polymerase chain reactions (PCRs) were designed on the basis of mycoplamalike organism (MLO) 16S rRNA sequences. The primer pair R16(I)F1/R1 specifically initiated amplification of 16S rDNA sequences among MLO strains in the MLO 16S rRNA group I, which includes aster yellows MLO and related strains; R16(III)F2/R1 specifically initiated amplifications in the MLO 16S rRNA group III, which includes peach X-disease MLO and related strains; and R16(V)F1/R1 specifically initiated amplification in the MLO 16S RNA group V, which includes elm yellows MLO and related strains [...]