articleTop 1% cited
Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants
Gene · 1982 · Vol. 18(3) · pp. 289–296
Alan M. Friedman✉(Harvard University)Sharon R. Long(Harvard University)Susan E. Brown(Harvard University)William J. Buikema(Harvard University)Frederick M. Ausubel(Harvard University)
Legume Nitrogen Fixing SymbiosisPlant nutrient uptake and metabolismBacteriophages and microbial interactionsBiologyCosmidEcoRIGeneticsBglIIAuxotrophyclone (Java method)MutantMolecular biologyPlasmid
MeSH terms
Base CompositionCloning, MolecularDNA Restriction EnzymesDrug Resistance, MicrobialEscherichia coliGenetic Complementation TestGenotypeMutationBacteriophage lambdaPlasmidsRhizobiumTetracyclineTransformation, Genetic
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References
Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis
Journal of Bacteriology · 1982 · 971 citations
A rapid boiling method for the preparation of bacterial plasmids
Analytical Biochemistry · 1981 · 3,310 citations
A complementation analysis of the restriction and modification of DNA in Escherichia coli
Journal of Molecular Biology · 1969 · 3,858 citations
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Journal of Molecular Biology · 1975 · 32,825 citations
A small cosmid for efficient cloning of large DNA fragments
Gene · 1980 · 824 citations
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