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Monoclonal antibody to cytokeratin for use in routine histopathology.

Journal of Clinical Pathology · 1984 · Vol. 37(9) · pp. 975–983

C. A. Makin✉(University College London)Lynda G. Bobrow(University College Hospital)Walter F. Bodmer(The Honourable Society of Lincoln's Inn)

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Abstract

CAM 5.2 is a murine monoclonal antibody, raised against the colon carcinoma cell line HT29, which recognises lower molecular weight intracellular cytokeratin proteins within secretory epithelia. Extensive indirect immunohistochemical studies have confirmed that this antibody stains formalin fixed (and freshly frozen) normal and malignant human tissue in a consistent manner. Reliable staining of conventionally processed pathological tissues provides more accurate identification and staging of human malignant epithelial diseases.

Skin and Cellular Biology Research Monoclonal and Polyclonal Antibodies Research Glycosylation and Glycoproteins ResearchCytokeratinMonoclonal antibodyImmunohistochemistryPathologyAntibodyStainingHistopathologyBiologyFrozen section procedureCarcinoma

MeSH terms

AnimalsAntibodies, MonoclonalAntibodies, NeoplasmCell LineColonic NeoplasmsFixativesFluorescent Antibody TechniqueHumansHybridomasImmunoglobulin GImmunoenzyme TechniquesKeratinsMice, Inbred BALB CStaining and LabelingMice

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References

The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells