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Confluent Monolayers of Cultured Human Fetal Retinal Pigment Epithelium Exhibit Morphology and Physiology of Native Tissue

Investigative Ophthalmology & Visual Science · 2006 · Vol. 47(8) · pp. 3612–3612

Arvydas Maminishkis✉(National Institutes of Health)Shan Chen(National Eye Institute)Stephen Jalickee(National Institutes of Health)Tina Banzon(National Institutes of Health)Guangpu Shi(National Eye Institute)Fei E. Wang(National Institutes of Health)Todd Ehalt(National Institutes of Health)Jeffrey A. Hammer(National Institutes of Health)Sheldon S. Miller(National Institutes of Health)

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Abstract

A new cell culture procedure has been developed that produces confluent primary hfRPE cultures with morphological and physiological characteristics of the native tissue. Epithelial polarity and function of these easily reproducible primary cultures closely resemble previously studied native human fetal and bovine RPE-choroid explants.

Barrier Structure and Function Studies Retinal Development and Disorders Connexins and lens biologyRetinal pigment epitheliumBiologyTight junctionElectron microscopeEpitheliumRetinalCell biologyMolecular biologyChemistryAnatomy

MeSH terms

Pigment Epithelium-Derived FactorCell SeparationElectrophysiologyEnzyme-Linked Immunosorbent AssayEye ProteinsFetusHumansMembrane PotentialsMicroscopy, ElectronNerve Growth FactorsPigment Epithelium of EyeBlotting, WesternSerpinsCytokinesCell Polarity

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