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Enzymatic assembly of DNA molecules up to several hundred kilobases

Nature Methods · 2009 · Vol. 6(5) · pp. 343–345
Daniel G. GibsonLei YoungRay-Yuan ChuangJ. Craig VenterClyde A. HutchisonHamilton O. Smith
CRISPR and Genetic EngineeringRNA and protein synthesis mechanismsGene Regulatory Network AnalysisDNA ligaseDNAExonucleaseDNA polymeraseDNA clampLigase chain reactionBiologyIn vitro recombinationComputational biologyGene

MeSH terms

Cloning, MolecularDNA-Directed DNA PolymeraseDNA, CircularDNA, RecombinantEscherichia coliGenesGenetic EngineeringGenetic TechniquesGenetic VectorsPlasmidsDNA LigasesGenomePhosphodiesterase IMycoplasma genitalium
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