Studies on the activities of tannins and related compounds from medicinal plants and drugs. I. Inhibitory effects on lipid peroxidation in mitochondria and microsomes of liver.

Abstract

The inhibitory effects of 25 tannins and related compounds on the lipid peroxidation induced by adenine 5'-diphosphate (ADP) and ascorbic acid in rat liver mitochondria, and on that induced in rat liver microsomes by ADP and nicotinamide adenine dinucleotide phosphate (NADPH), were determined. All of the tannins, except for some polyphenols of low molecular weight and methylated polyphenols, showed significant inhibition in these two systems at the concentration of 1μg/ml. Almost complete inhibition of the lipid peroxidation in the two systems was shown by some ellagitannins such as pedunculagin, isoterchebin, etc., at the dose of 5μg/ml. Marked differences of the inhibitory activities were observed among the tannins depending on the tannin structure, including the stereoisomerism in the monomer of condensed tannin, and on the experimental system used. The inhibitory effects of most of the hydrolyzable tannins were higher than those of the condensed tannins in both systems. Some monomeric polyphenols, represented by (-)-epigallocatechin gallate, showed inhibitory effects stronger than those of the condensed tannins in these systems, particularly on the lipid peroxidation induced by ADP and NADPH. The inhibitory effects of these tannins in both systems were very much stronger than that of α-tocopherol.