articleTop 1% cited

Two-photon imaging to a depth of 1000 µm in living brains by use of a Ti:Al_2O_3 regenerative amplifier

Optics Letters · 2003 · Vol. 28(12) · pp. 1022–1022

Patrick Theer✉(Max Planck Institute for Medical Research)Mazahir T. Hasan(Max Planck Society)Winfried Denk(Max Planck Institute for Medical Research)

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Abstract

It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence.

Advanced Fluorescence Microscopy Techniques Nonlinear Optical Materials Studies Photoreceptor and optogenetics researchFemtosecondOpticsTwo-photon excitation microscopyFluorescenceRegenerative amplificationFluorescence-lifetime imaging microscopyExcitationPhoton countingPhotonMaterials science

MeSH terms

Aluminum OxideAnimalsBlood VesselsBrainCerebral CortexCerebrovascular CirculationDextransIndicators and ReagentsLuminescent ProteinsMice, TransgenicMicroscopy, FluorescencePeriaqueductal GrayTitaniumFluorescein-5-isothiocyanatePhotons

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Deep tissue two-photon microscopy