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The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen
Biochemical Journal · 1975 · Vol. 145(1) · pp. 93–103
Abstract
The chemical nature of the important new chromogen ABTS [2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid)] is described together with an account of the redox and spectroscopic properties of the system ABTS--H2O2--peroxidase. Keq. is calculated and a study of the steady-state kinetics over a whole range of substrate concentrations is reported. By using novel methods of kinetic analysis, an interpretation of the results is given which requires some extension of the classical peroxidase mechanism.
bioluminescence and chemiluminescence researchProtein Interaction Studies and Fluorescence AnalysisSpectroscopy and Quantum Chemical StudiesABTSPeroxidaseChemistryKineticsSteady state (chemistry)Substrate (aquarium)Enzyme kineticsOrganic chemistryStereochemistryEnzyme
MeSH terms
Hydrogen PeroxideIndicators and ReagentsKineticsMathematicsModels, ChemicalOxidation-ReductionPeroxidasesSpectrophotometrySulfonic AcidsThiazolesBenzothiazoles
Funding
- Medical Research Council
Citations
890
FWCI
2.98
field-weighted impact
References
20
Percentile
91%
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