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Analytical Chemical Studies on Amino Sugars. II. Deterimation of Hexosamines using 3-Methyl-2-benzothiazolone Hydrazone Hydrochloride
Chemical and Pharmaceutical Bulletin · 1969 · Vol. 17(7) · pp. 1505–1510
Akio Tsuji✉(Showa Pharmaceutical University)Toshio Kinoshita(Showa Pharmaceutical University)Masanori Hoshino(Showa Pharmaceutical University)
Abstract
A novel method for determination of hexosamines was established utilizing 3-methyl-2-benzothiazolone hydrazone hydrochloride (MBTH) and ferric chloride. This method was highly sensitive and specific. All the procedure was carried out in mildly acidic aqueous solution at room temperature. The hexosamine content of chondroitin sulfates and hyaluronic acid estimated by this method showed good agreement with that calculated from nitrogen contents of the polysaccharides. Moreover, only 2 hr of hydrolysis with 2N hydrochloric acid was sufficient for the assay of these mucopolysaccharides.
Proteoglycans and glycosaminoglycans researchOcular Surface and Contact LensGlycosylation and Glycoproteins ResearchChemistryHexosaminesHydrochlorideHydrochloric acidHydrazoneHydrolysisChlorideFerricOrganic chemistryChromatography
MeSH terms
ColorimetryGlycosaminoglycansHexosaminesIndicators and ReagentsMethodsSpectrophotometry
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