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Simple and rapid method for isolating large plasmid DNA from lactic streptococci
Applied and Environmental Microbiology · 1983 · Vol. 46(3) · pp. 549–552
Abstract
A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.
Streptococcal Infections and TreatmentsInfective Endocarditis Diagnosis and ManagementMicrobial infections and disease researchPlasmidTransformation (genetics)DNAPlasmid preparationBiologyBacteriaMicrobiologyRestriction mapGeneticsGene
MeSH terms
DNA, BacterialMethodsPlasmidsLactococcus lactis
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