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Simple method for identification of plasmid-coded proteins

Journal of Bacteriology · 1979 · Vol. 137(1) · pp. 692–693
Aziz SancarAdelle M. HackW. Dean Rupp

Abstract

Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA.

Bacterial Genetics and BiotechnologyAntibiotic Resistance in BacteriaRNA and protein synthesis mechanismsPlasmidBiologyEscherichia coliPlasmid preparationDNAMolecular biologyGeneticsChromosomeCircular bacterial chromosomeGene

MeSH terms

Bacterial ProteinsDNA ReplicationDNA, BacterialEscherichia coliPlasmidsUltraviolet Rays
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